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eclipse ti e c2 confocal laser scanning microscope  (Nikon)


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    Nikon eclipse ti e c2 confocal laser scanning microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E C2 Confocal Laser Scanning Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti+e+microscope/C2%2B/pmc12811640-78-16-23
    Average 99 stars, based on 1 article reviews
    eclipse ti e c2 confocal laser scanning microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7"

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2025.100335

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.
    Figure Legend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Techniques Used: Staining, Mutagenesis, Fluorescence, Microscopy

    Related Articles

    Microscopy:

    Article Title: Sinorhizobium meliloti FcrX coordinates cell cycle and division during free-living growth and symbiosis by a ClpXP-dependent mechanism.
    Article Snippet: Stacks were transformed into maximum intensity projections using ImageJ software. .. Time- lapse experiments were performed on depleted cells deposited on agarose/YEB (with appropriate antibiotics and inducers) and observed every 10 min (up to 16 h) on a Nikon Eclipse Ti E microscope equipped with a Yokogawa CSU- X1- A1 spinning disk system. ..

    Article Title: Mechanobiological regulation of T cells via transient viscoelastic microfluidic confinement.
    Article Snippet: In each experiment, cells were centrifuged at 5000 rpm for 5 minutes at 24 °C, filtered through a strainer with a pore size of 40 μm (Corning, New York, NY, USA), centrifuged, and re-suspended in a fresh cell medium. .. Bright-field imaging of cells was performed using a Y5.1 high-speed camera (Motion Pro, Niederoenz, Switzerland) mounted on an inverted Eclipse Ti-E microscope (Nikon, Zurich, Switzerland) using a 30×, 0.45 NA S Plan Fluor objective (Nikon, Zurich, Switzerland), and ultra-high power UHP-T-560-DI-DF LED (Prizmatix, Holon, Israel). ..

    Article Title: Actin-dependent α-catenin oligomerization contributes to adherens junction assembly.
    Article Snippet: .. In brief, cells were imaged (in IP-buffer, Fig. 2e, or in L-15 media with 10% FBS, Fig. 8 and Supplementary Fig. 6) by an Eclipse Ti-E microscope (Nikon, Melville, NY) at RT or 37°C controlled with Nikon’s NIS-Elements software. ..

    Article Title: The Pro-Angiogenic Potential of Periodontal Ligament Stem Cells and Dental Pulp Stem Cells: A Comparative Analysis.
    Article Snippet: Briefly, HMEC-1 were plated on growth factor-reduced Matrigel (Corning, NY, USA) in μ-plate 96-well 3D (IBIDI GmBh, Gräfelfing, Germany), utilizing conditioned medium of DPSCs and PDLSCs at 100% and 50% with α-MEM. .. Cell organization in Matrigel was acquired after 13 h using a Nikon Eclipse Ti E microscope using a Nikon Plan 10 ×/0.10 objective (Nikon Instruments, Amsterdam, The Netherlands). ..

    Article Title: The Pro-Angiogenic Potential of Periodontal Ligament Stem Cells and Dental Pulp Stem Cells: A Comparative Analysis
    Article Snippet: Briefly, HMEC-1 were plated on growth factor-reduced Matrigel (Corning, NY, USA) in μ-plate 96-well 3D (IBIDI GmBh, Gräfelfing, Germany), utilizing conditioned medium of DPSCs and PDLSCs at 100% and 50% with α-MEM. .. Cell organization in Matrigel was acquired after 13 h using a Nikon Eclipse Ti E microscope using a Nikon Plan 10×/0.10 objective (Nikon Instruments, Amsterdam, The Netherlands). ..

    Article Title: Mechanobiological regulation of T cells via transient viscoelastic microfluidic confinement
    Article Snippet: In each experiment, cells were centrifuged at 5000 rpm for 5 minutes at 24 °C, filtered through a strainer with a pore size of 40 μm (Corning, New York, NY, USA), centrifuged, and re-suspended in a fresh cell medium. .. Bright-field imaging of cells was performed using a Y5.1 high-speed camera (Motion Pro, Niederoenz, Switzerland) mounted on an inverted Eclipse Ti-E microscope (Nikon, Zurich, Switzerland) using a 30×, 0.45 NA S Plan Fluor objective (Nikon, Zurich, Switzerland), and ultra-high power UHP-T-560-DI-DF LED (Prizmatix, Holon, Israel). ..

    Article Title: Medicago Mting1 Mting2 double knockout mutants are extremely dwarfed and never flower implicating essential MtING functions in growth and flowering
    Article Snippet: .. Confocal laser scanning microscopy images were captured on an Eclipse Ti-E microscope with a 40 × S Plan Fluor ELWD objective lens (Nikon, Japan), CSU-X1 confocal scanner unit (Yokogawa, Japan), ALC-500 laser unit and Zyla sCMOS camera (Andor, UK). .. GFP and mCherry were excited using a laser light wavelength of 488 nm and 561 nm, respectively, and images captured with a 3035B and TX Red 4040B filter (Semrock, USA).

    Article Title: Energy injection in an epithelial cell monolayer indicated by negative viscosity
    Article Snippet: .. All imaging was performed on an Eclipse Ti-E microscope (Nikon Instruments) with a 10× numerical aperture 0.5 objective (Nikon) and an Orca Flash 4.0 digital camera (Hamamatsu) using Elements Ar software (Nikon). ..

    Imaging:

    Article Title: Mechanobiological regulation of T cells via transient viscoelastic microfluidic confinement.
    Article Snippet: In each experiment, cells were centrifuged at 5000 rpm for 5 minutes at 24 °C, filtered through a strainer with a pore size of 40 μm (Corning, New York, NY, USA), centrifuged, and re-suspended in a fresh cell medium. .. Bright-field imaging of cells was performed using a Y5.1 high-speed camera (Motion Pro, Niederoenz, Switzerland) mounted on an inverted Eclipse Ti-E microscope (Nikon, Zurich, Switzerland) using a 30×, 0.45 NA S Plan Fluor objective (Nikon, Zurich, Switzerland), and ultra-high power UHP-T-560-DI-DF LED (Prizmatix, Holon, Israel). ..

    Article Title: Mechanobiological regulation of T cells via transient viscoelastic microfluidic confinement
    Article Snippet: In each experiment, cells were centrifuged at 5000 rpm for 5 minutes at 24 °C, filtered through a strainer with a pore size of 40 μm (Corning, New York, NY, USA), centrifuged, and re-suspended in a fresh cell medium. .. Bright-field imaging of cells was performed using a Y5.1 high-speed camera (Motion Pro, Niederoenz, Switzerland) mounted on an inverted Eclipse Ti-E microscope (Nikon, Zurich, Switzerland) using a 30×, 0.45 NA S Plan Fluor objective (Nikon, Zurich, Switzerland), and ultra-high power UHP-T-560-DI-DF LED (Prizmatix, Holon, Israel). ..

    Article Title: Energy injection in an epithelial cell monolayer indicated by negative viscosity
    Article Snippet: .. All imaging was performed on an Eclipse Ti-E microscope (Nikon Instruments) with a 10× numerical aperture 0.5 objective (Nikon) and an Orca Flash 4.0 digital camera (Hamamatsu) using Elements Ar software (Nikon). ..

    Software:

    Article Title: Actin-dependent α-catenin oligomerization contributes to adherens junction assembly.
    Article Snippet: .. In brief, cells were imaged (in IP-buffer, Fig. 2e, or in L-15 media with 10% FBS, Fig. 8 and Supplementary Fig. 6) by an Eclipse Ti-E microscope (Nikon, Melville, NY) at RT or 37°C controlled with Nikon’s NIS-Elements software. ..

    Article Title: Energy injection in an epithelial cell monolayer indicated by negative viscosity
    Article Snippet: .. All imaging was performed on an Eclipse Ti-E microscope (Nikon Instruments) with a 10× numerical aperture 0.5 objective (Nikon) and an Orca Flash 4.0 digital camera (Hamamatsu) using Elements Ar software (Nikon). ..

    Confocal Laser Scanning Microscopy:

    Article Title: Medicago Mting1 Mting2 double knockout mutants are extremely dwarfed and never flower implicating essential MtING functions in growth and flowering
    Article Snippet: .. Confocal laser scanning microscopy images were captured on an Eclipse Ti-E microscope with a 40 × S Plan Fluor ELWD objective lens (Nikon, Japan), CSU-X1 confocal scanner unit (Yokogawa, Japan), ALC-500 laser unit and Zyla sCMOS camera (Andor, UK). .. GFP and mCherry were excited using a laser light wavelength of 488 nm and 561 nm, respectively, and images captured with a 3035B and TX Red 4040B filter (Semrock, USA).



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    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
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    Image Search Results


    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy